▶️DRAGEN 16S Plus App Documentation

Overview

Summary:

The DRAGEN 16S Plus application is a rapid, kmer-based informatics solution designed for microbial classification and community profiling from mixed flora and metagenomic sample types. The app delivers easy-to-use, powerful secondary analysis of Illumina 16S sequencing data, with workflows for read QC (optional), taxonomic classification, result filtering (optional), and reporting. It also supports custom database analysis.

Input files:

• FASTQ files

• Custom database FASTA file (if applicable)

Demo Data:

The DRAGEN 16S Plus Demo Project includes 21 samples prepared using the Illumina 16S metagenomic sequencing library preparation protocol. An example custom reference sequence FASTA file is also included.

Analysis Pipeline:

1. Read QC (optional)

2. Taxonomic classification

3. Result filters (optional read count threshold)

4. Reporting

Output files:

• Analysis-level outputs: XLSX, CSV, HTML, ZIP

• Sample-level outputs: JSON, CSV, HTML, TXT.GZ

Important Notes:

DRAGEN 16S Plus is a secondary analysis tool for research use only. Further interpretation, statistical analysis, and downstream analysis of results may be necessary.

For questions on this application please contact Illumina Technical Support at [e-mail].

For Research Use Only. Not for use in diagnostic procedures.