🚩Release notes

DRAGEN 16S Plus app version 1.0.0

Initial release.

Component versions

• Reference database: Refseq-RDP-v1 (full-length NCBI RefSeq 16S rRNA database supplemented by RDP)

• Analysis Pipeline version: 0.7.0

• DRAGEN version: 4.4.4-19

Known Issues

• Analysis fails when launched with a single-read Fastq file

• When Read QC is disabled, the "Classification Statistics" table values and "Top Results by Taxonomic Level” barplots are missing from sample HTML report files

• When reading Biosamples from a Project, Fastq files for Biosamples sharing the same sample name prefix before the first underscore are merged. For example, Fastq files for Biosamples PREFIX_001 and PREFIX_002 will be merged and reported as a single PREFIX sample. To avoid this error, ensure that sample names are unique before the first underscore, replace underscores with a hyphen, or provide Biosample input from a list

Known Limitations

• 100 basepairs minimum read length

• 500 million basepairs maximum for custom database specification

• Species level classification from 16S amplicon data is generally less reliable than genus level classification

• Summary dendrogram chart may not be rendered

• Summary species rarefaction curve chart may not be rendered

• Sample-level species rarefaction curve chart may not be rendered